Thesis LIGNOCELLULOSIC WASTE VALORISATION STRATEGY THROUGH ENZYME AND BIOGAS PRODUCTION
Date
2017
Authors
WYMAN SAN MARTÍN, VALENTINA PAZ
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Abstract
Los residuos lignocelulósicos son, por lo general, pre-tratados para facilitar la etapa de hidrólisis durante el proceso de digestión anaerobia. Se evaluó un proceso secuencial consistente en la fermentación de sustrato sólido, realizada por hongos de putrefacción blanca, y digestión anaerobia en rastrojo de maíz para producir enzimas ligninolíticas y biogás. La producción de enzimas fue cuantificada cada 3 días durante un mes a 30 °C, y se comparó en tres cepas de hongo y dos tamaños de partículas del residuo. Dentro de los principales resultados, se obtuvo una mayor actividad enzimática de lacasa con la cepa Pleurotus eryngii en comparación con las cepas Pleurotus ostreatus y Trametes versicolor. Además, esta actividad mejoró en un 16% cuando se utilizó cobre como inductor enzimático. Por otro lado, la mayoría de las condiciones estudiadas mostraron una disminución en la producción máxima de biogás comparada con el residuo sin tratar. La adición de cobre disminuyó la producción de biogás en un 20%. A pesar de lo anterior, Pleurotus eryngii mostró resultados prometedores que permitieron un aumento del 19% en la producción de biogás y altos valores de producción de enzimas.
Lignocellulosic wastes are generally pre-treated to facilitate the hydrolysis stage during the anaerobic digestion process. A sequential process consisting of solid substrate fermentation carried out by white rot fungi and anaerobic digestion was evaluated on corn stover to produce ligninolytic enzymes and biogas. The enzyme production was quantified every 3d for a month at 30°C, and three fungal strains and two particle sizes of waste were compared. Of the main outcomes, Pleurotus eryngii produced the highest laccase enzyme activity compared with Pleurotus ostreatus and Trametes versicolor. Furthermore, this activity was improved by 16% when copper was used as an enzyme inducer. On the other hand, most of the conditions studied showed a decrease in maximum biogas production compared with untreated waste, the addition of copper decreased biogas production by 20%. Despite the above, Pleurotus eryngii showed promising results allowing a 19% increase of biogas production and high enzyme production values.
Lignocellulosic wastes are generally pre-treated to facilitate the hydrolysis stage during the anaerobic digestion process. A sequential process consisting of solid substrate fermentation carried out by white rot fungi and anaerobic digestion was evaluated on corn stover to produce ligninolytic enzymes and biogas. The enzyme production was quantified every 3d for a month at 30°C, and three fungal strains and two particle sizes of waste were compared. Of the main outcomes, Pleurotus eryngii produced the highest laccase enzyme activity compared with Pleurotus ostreatus and Trametes versicolor. Furthermore, this activity was improved by 16% when copper was used as an enzyme inducer. On the other hand, most of the conditions studied showed a decrease in maximum biogas production compared with untreated waste, the addition of copper decreased biogas production by 20%. Despite the above, Pleurotus eryngii showed promising results allowing a 19% increase of biogas production and high enzyme production values.
Description
Catalogado desde la version PDF de la tesis.
Keywords
ESTRATEGIA DE VALORIZACION , PRODUCCION DE BIOGAS , RESIDUOS DE MAIZ , RESIDUOS LIGNOCELULOSICOS
